The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30–84

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The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30–84

Enhanced production of 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) by the biological control strain Pseudomonas chlororaphis 30-84 derivative 30-84O* was shown previously to promote cell adhesion and alter the three-dimensional structure of surface-attached biofilms compared to the wild type. The current study demonstrates that production of 2-OH-PCA promotes the release of extracellular D...

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Pip , a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis 1 PCL 1391 2 Running title : a new regulator of phenazine synthesis in Pseudomonas 3 4

3 Present address: Crucell Holland B.V., PO Box 2048, 2301 CA Leiden, The Netherlands 11 12 13 *Corresponding author. 14 Tel: +31 71 524 3974 15 Fax: +31 71 524 8702 16 E-mail address: [email protected] 17 AC CE PT ED Copyright © 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.00893-06 JB Accepts, published onli...

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Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa

In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA...

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A kinetic model for phenazine-1-carboxylic acid production by pseudomonas sp. M18G

The development of optimal bacterial strains for industrial scale of fermentation would benefit from a quantitative model of rate of gene expression from DNA to RNA, from DNA to protein, and ultimately from DNA to the metabolites. In this study, we developed such a model for phenazine-1-carboxylic acid (PCA) biosynthesis in Pseudomonas sp. strain M18G. The PCA biosynthesis is controlled by anop...

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Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391.

Production of the secondary metabolite phenazine-1-carboxamide (PCN) by Pseudomonas chlororaphis PCL1391 is crucial for biocontrol activity against the phytopathogen Fusarium oxysporum f. sp. radicis lycopersici on tomato. Regulation of PCN production involves the two-component signalling system GacS/GacA, the quorum-sensing system PhzI/PhzR and the regulator PsrA. This paper reports that a fun...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2016

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0148003